Afm Phase Lag Mapping For Protein-Dna Oligonucleotide Complexes [An Article From: Analytica Chimica Acta]

Afm Phase Lag Mapping For Protein-Dna Oligonucleotide Complexes [An Article From: Analytica Chimica Acta]

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    This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

    Description:
    Atomic force microscope phase lag imaging of protein-DNA oligonucleotide complexes has been performed to visualize the immobilized oligonucleotides on the protein surface. In normal sample conditions, neither the topographic nor phase lag images show any discriminate signals for the immobilized oligonucleotides. Use of a highly humid incubator, controls the surface humidity of the sample. Thereby, the phase lag image reveals the oligonucleotide location by the local difference of tip adhesion distribution. The resultant phase lag image shows extremely strong signals in the center of the protein surface, indicating the location of the oligonucleotides with resolution better than 20nm. The signal frequency was strongly influenced by the used oligonucleotide concentration in the range 5nM-50@mM.

      Author

      J.M. Kim

      Author

      H.S. Jung

      Author

      J.W. Park

      Author

      H.Y. Lee

      Author

      T Kawai

      Binding

      Digital

      Format

      HTML

      Label

      Elsevier

      Manufacturer

      Elsevier

      ProductGroup

      Book

      ProductTypeName

      ABIS_EBOOKS

      PublicationDate

      2004-11-08

      Publisher

      Elsevier

      Studio

      Elsevier

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